This BIORESEARCH TECHNOLOGY UPDATE is to inform you about the use of the Warner Instruments TC-344B Dual Channel Temperature Controller to determine the relationship between adenomatous polyposis coli (APC) protein with microtubule (MT) ends and its binding partner EB1.

The TC-344B Temperature Controller was used to maintain the temperature of the Warner SH-27B Inline Solution Heater and Warner RC-30 Imaging Chamber during drug perfusion of living canine kidney epithelial cells and mouse primary fibroblasts.

Using the APC-deficient cell line and small inhibitory RNA inhibition of EB1 expression, the study demonstrated that the association of endogenous APC with MT ends correlates directly with their increased growth stability, that this can occur independently of its association with EB1, and that APC and EB1 can associate with MT plus ends by distinct mechanisms.

The dynamic MT cytoskeleton is critical to cell functions such as cell division, directed cell migration, the immune response, and neuronal development.

Click on the title below to download the article.

Adenomatous Polyposis Coli on Microtubule Plus Ends in Cell Extensions Can Promote Microtubule Net Growth with or without EB1
Katsuhiro Kita,^*Torsten Wittmann,^*,†Inke S. Nathke,^‡Clare M. Waterman-Storer^*
Molecular Biology of the Cell May 2006; Vol. 17:2331-2345
^*Department of Cell Biology, The Scripps Research Institute, La Jolla , CA 92037
^‡Division of Cell and Developmental Biology, School of Life Sciences , University of Dundee , Dundee DD1 5EH , United Kingdom
^†Present address: Department of Cell and Tissue Biology, University of California at San Francisco School of Dentistry, 513 Parnassus Ave , HSW-616, Box 0512 , San Fransicso , CA 94143

A Warner Instruments TC-344B Dual Channel Temperature Controller was used to maintain temperature of drug perfusion during live cell imaging in this study.

Information on this product can be found on page 94 of the Warner Instruments 2007 Cell Biology and Electrophysiology Catalog or by clicking on the following link:

TC-344B Dual Channel Temperature Controller

• Slow ramped DC power for quiet operation
• Single control temperature adjustment
• Manually controlled DC output option
• Ambient to 50°C operation
• Three feedback loop speed options
• Independent bath temperature monitoring

Please contact newsletter@harvardapparatus.com with questions, comments or concerns.